Journal: Experimental eye research

Article Title: Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork.

doi: 10.1016/j.exer.2022.109235

Figure Lengend Snippet: Figure 1. Authentication of primary cultured rat trabecular meshwork (TM) cells. (A) Isolation and proliferation of primary TM cells observed under light microscope. a. Tissue scraps cut from corneoscleral rims. Co: cornea; Ir: iris. Blue triangles indicate the location where TM is supposed to reside. b. Primary TM cells started to crawl out of tissue scraps (indi cated by *) and adhere (indicated by black triangles). c. The attached cells gradually formed “clusters” and became contact-inhibited. d-f. Passage 3 TM cells presented typical spindle-like “fibroblast” phenotype and became cobblestone-like once confluent. Scale bars = 100 μm. (B) Immunofluorescence staining of cultured TM cells with anti-fibronectin, anti-laminin, anti-neuron-specific enolase (NSE), anti-factor- Ⅷ-related antigen (FⅧRAg) antibodies. Scale bar = 100 μm. (C–E) Cells were treated with 200 nM dexamethasone (Dex) for 7 days. Immunohistochem istry staining showed that over fifty percent of TM cells were myocilin-positive (the whole cell was yellow-stained) and was statistically different to that of vehicle control. Western blotting with anti- myocilin antibody showed a doublet at 55 kDa for the Dex-treated group. MYOC: myocilin. Scale bar = 300 μm. (F) Cells were treated with 100 nM Dex or ethanol control for 7 days and dyed with phalloidin. Cross-linked actin networks (CLANs) were observed and indicated by white triangles. Scale bars = 100 μm.

Article Snippet: Expression of fibronectin (M00564-3, BOSTER, China, 1:50), laminin (A03522, BOSTER, China, 1:50), neuron-specific enolase D. Xu et al.

Techniques: Cell Culture, Isolation, Light Microscopy, Immunofluorescence, Staining, Control, Western Blot

Journal:

Article Title: In Vivo Expression of Putative LMX1B Targets in Nail-Patella Syndrome Kidneys

doi:

Figure Lengend Snippet: Characteristics of Antibodies Used in the Study

Article Snippet: Rabbit anti-fibronectin , Fibronectin , 1/80 , Novotec, Lyon, France.

Techniques: